Abstract 100: Human Mesenchymal Stromal Cells Engineered to Overexpress PDGF-B Using CRISPR/Cas9/rAAV6-based Tools Improve Wound Healing
نویسندگان
چکیده
PURPOSE: Mesenchymal Stromal Cells (MSCs) are a promising source for cell therapy due to their proregenerative and immunomodulatory effects. These properties can be enhanced by engineering MSCs to overexpress and secrete growth factors favorable to wound healing processes. Here, we aimed to develop a platform for targeted gene insertion in human bone marrow (hBM)-MSCs that utilizes CRISPR/Cas9 nuclease and recombinant adeno-associated virus serotype 6 (rAAV6) to deliver a designed homologous recombination-based repair template. Using this method, we engineered hBM-MSCs to overexpress and secrete soluble platelet derived growth factor B (PDGF-B) and examined the ability of this cell line to promote wound healing.
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